Journal: Cancers

Article Title: Oncogenic and Stemness Signatures of the High-Risk HCMV Strains in Breast Cancer Progression

doi: 10.3390/cancers14174271

Figure Lengend Snippet: Replication of B544 and B693 strains in MRC5 cultures, and the appearance of morphologically distinct cells following the infection of HMECs with these high-risk strains. ( A ) Time-course of the viral titer in the supernatant of MRC5 infected with the strains HCMV-B544 and HCMV-B693, as measured by IE1-qPCR. ( B ) Confocal microscopic images of HCMV-IE1 and pp65 staining in HMECs infected with HCMV-B544 and HCMV-B693 (day 1 post-infection). Uninfected HMECs were used as controls. Nuclei were counterstained with DAPI; magnification ×63, scale bar 10 μm. ( C ) HMECs time-course infection with HCMV-B544 and HCMV-B693 strains (MOI = 1). Magnification ×100, scale bar 100 μm. Uninfected HMECs were used as a control. ( D ) Presence of giant cells with blastomere-like morphology (1 and 6), mesenchymal cells (4 and 7), lipid droplet-packed cells (3, 8, and 9), cells displaying multiple nuclei (2) as well as cell budding (4, 5, and 6), and cells with filopodia protrusions (9) in CTH-B544 and CTH-B693 cells. The inverted light microscope scale bar represents 100 µm; magnification ×200.

Article Snippet: HMECs (A10565, Life Technologies, Carlsbad, CA, USA), CTH cells, MDA-MB231 as well as MCF7 (Institut Hiscia, Arlesheim, Switzerland), and MRC5 (RD-Biotech, Besançon, France) were cultured as previously described [ ].

Techniques: Infection, Staining, Light Microscopy

Journal: Nature Communications

Article Title: Multifunctional hydrogel nano-probes for atomic force microscopy

doi: 10.1038/ncomms11566

Figure Lengend Snippet: ( a ) Contact mode height images, with fixed 3 nN contact forces, for human fibroblast cells (MRC-5) in PBS imaged by a commercial silicon probe (first row) and a fabricated hydrogel probe (second row) at various scan rates. All scale bars are 5 μm. ( b ) Height profiles along the A–A′ and B–B′ lines in a . ( c ) Contact mode height images, with fixed 300 nN contact force, for MRC-5 cells in PBS sequentially imaged by using hydrogel (first), silicon (second) and hydrogel (third) probes at a scan rate of 1 Hz. All scale bars are 5 μm. ( d ) Height profiles along the A–A′ line in c .

Article Snippet: Human breast cancer cells (MCF-7, Sigma), human fibroblast cells (MRC-5, Japanese Collection of Research Bioresources Cell Bank; JCRB Cell Bank) and HeLa cells (ATCC CCL-2, ATCC) were used in experiments.

Techniques: